Total protein concentration was higher in D1 and D2 when compared with C. These data are consistent with the data obtained in children with type 1 diabetes[13,14] and adults with type 2 diabetes.[16] Our data showed significantly higher activities of salivary AST and ALT in D1 compared with C. Similar data concerning adult subjects were presented by Musumeci et al,[23] Al-Rubee et al,[24] and Verma et al.[25] However, Cinquini et al[26] did not notice any significant differences in the level of salivary AST and ALT in children with type 1 diabetes compared with the control group. The gene discussed is GPT; the disease is type 1 diabetes mellitus.