The number of differently methylated genes increased to 10 (BNIP3, BRCA1, CCND1, HTATIP2, ITGAV, NFKB1, PIK3R1, PRDM16, PTX3 and TAP2) between ESCC and healthy control tissues (Table 3). The gene discussed is BRCA1; the disease is esophageal squamous cell carcinoma.