TGFB3 and metabolic dysfunction-associated steatotic liver disease: In another study with 1,322 healthy subjects without other risk factors, serum TGF-β3 levels was significantly higher in those who developed NAFLD than in those who did not (mean 554 vs. 285 pg/ml; P = 0.002) after 4 years of follow-up; and as the TGF-β3 tertiles increased, there was a significant increase in NAFLD incidence (6.3, 38.0, and 55.7% for the first, second, and third tertiles, respectively; P < 0.05) (50).