Next, when podocytes were cultured at the nonpermissive temperature of 37°C and infection with DACH1 or control plasmid, western blotting analysis revealed that the expression of p53 and p21 elevated in DACH1-overexpressing group, in contrast, the cyclin D1 protein significantly decreased (Figure 8D, 8F). This evidence concerns the gene CDKN1A and infection.