Finally, in order to assess the effect of TAK-659 in CLL cells being stimulated by different players from the microenvironment we pre-treated CLL cells with TAK-659 1 μM for 1 hour followed by co-culture with BMSC, CD40L and CpG ODN for 15 minutes and subsequent BCR crosslinking for 5 additional minutes. This evidence concerns the gene CD40LG and B-cell chronic lymphocytic leukemia.