No statistically significant difference was observed between dose effect curves for anti-IgM or co-culture alone compared to CLL cells in suspension although LD50 for CLL cells in suspension was higher (TAK-659 LD50 for CLL cells with anti-IgM: 22.04 μM [95%CI 8.72–55.69] vs. TAK-659 LD50 for CLL cells in co-culture: 31.89 μM [95%CI 19.93–51.05], P > 0.05) (Figure 3B). This evidence concerns the gene CD40LG and B-cell chronic lymphocytic leukemia.