To assess the effect of PD on TLR2 expression, SDS-soluble fractions from the anterior cingulate cortex were immunoblotted for TLR2, along with α-synuclein to assess PD pathology, and markers for specific brain cell types (NeuN for neurons, Iba1 (combined data from both the TBS and SDS-soluble fractions) and HLA-DR for microglia and activated microglia, respectively). This evidence concerns the gene SNCA and Parkinson disease.