In addition, overexpression of TMIGD3 i1 and A3AR, but not TMIGD3 i3, inhibited migration of SJSA-1 and KHOS/NP cells (Fig. 4e), as well as tumour growth of SJSA-1 cells (Fig. 4f), thereby suggesting that TMIGD3 i1 and A3AR suppress malignant properties of OS cells in vitro and in vivo. This evidence concerns the gene TMIGD3 and neoplasm.