The number of methylation probes included in the model could be reduced to 3 probes (RPS6KA2 cg175012010, cg09349128, cg25114611); however, this led to a reduction in specificity for IBD and a higher misclassification rate (AUC=0.87, sensitivity=0.906, specificity=0.542, misclassification rate=0.243). Here, RPS6KA2 is linked to inflammatory bowel disease.