Interestingly, upregulated miRNAs including let-7b, miR-146a, miR-103, miR-125a-5p and miR-342-3p were found to be present in the EVs isolated from human tissue samples affected with prion diseases,124 suggesting thereby that PRNP-infected EVs also mediate the pathogenesis of prion disease through their contents in addition to spreading of PRNP. The gene discussed is PRNP; the disease is prion disease.