We then compared the proliferation inhibitory effect of LDM upon 48-h treatment across these cell lines, and found that LDM was relatively more potent in HCT116 and SW620 cells (with IC50 being 0.67 and 0.97 nM, respectively) among the tested cells, indicating that cancer cells with higher EZH2 expression were more susceptible to LDM-induced cytotoxicity (Figure 2b). The gene discussed is EZH2; the disease is cancer.