Univariate analysis of an initial cohort of 129 all AJCC stage melanomas further revealed decreased or loss of epidermal AMBRA1 expression was significantly associated with decreased disease-free survival, with stratification for AJCC stage I disease, additionally revealing epidermal AMBRA1 expression as a putative biomarker of disease progression [Figure 2C; (32)]. This evidence concerns the gene AMBRA1 and melanoma.