In addition to studies documenting increased NF-κB at the protein level and evidence of its activation in AD, Rao et al. (2012) reported hypomethylation of the NF-κB promoter CpG region and a concomitant increase in mRNA expression of p65 and p50 subunits in the frontal cortex of those with AD relative to age-matched controls despite hypermethylation of DNA at the global level. This evidence concerns the gene NFKB1 and Alzheimer disease.