The correlation between the expression of TREX1, clinical manifestation, and prognosis of osteosarcoma in patients as well as biological characteristics of osteosarcoma stem cell was investigated in the paper, the relationship between the expression of TREX1 and osteosarcoma stem cells was clarified, so as to explore the correlation between the expression of TREX1 and the metastasis risk and clinical prognosis of patients with osteosarcoma. The gene discussed is TREX1; the disease is osteosarcoma.