The expressions of TREX1 mRNA and protein in CD133− cells of human osteosarcoma MNNG/HOS cells were significantly higher than those of CD133+ cells, suggesting that TREX1 was lowly expressed in osteosarcoma stem cells and highly expressed in non-osteosarcoma stem cells, indicating that the expressions of TREX1 gene and protein were closely related to osteosarcoma cell stem cell properties, also indicated that the expression of TREX1 could indicate the stem cell characteristics of osteosarcoma cells, illustrating the proliferation and metastasis of osteosarcoma cells. Here, TREX1 is linked to osteosarcoma.