In order to investigate the in vivo antitumor effects of ASMq on the U27 tumor mouse model, this study focused on the tumor inhibition rate, tumor histopathology, and TGF-β1 and TNF-a protein expression levels of tumor tissues obtained from the U27 tumor model group, CTX group, and low-, medium-, and high-dose of ASMq groups. The gene discussed is TGFB1; the disease is neoplasm.