Univariate analysis revealed that presence of macroscopic vascular invasion (P<0.001), higher pre-treatment LDH level (P<0.001) (Figure 3A), higher AFP level (P=0.001), advanced Child-Pugh class (P=0.004), larger tumor size (P<0.001), advanced T category (P<0.001), presence of concomitant treatment (P=0.001), presence of dose reduction (P=0.045) and advanced BCLC stage (P=0.032) were significantly associated with the OS (Table 3). This evidence concerns the gene AFP and neoplasm.