TP53 and neoplasm: To investigate the regulation of glycolysis gene expression in the context of a MCPyV-transformed cell line, IMR90 cells were serially transduced with retroviral constructs expressing a dominant-negative form of p53 (p53DD), telomerase reverse transcriptase (hTERT), and a tumor-derived form of MCPyV early-region (ER) that expresses ST plus truncated LT to generate p53DD-hTERT-ER (PHE) cells.