LGR5 and colorectal carcinoma: After 42 days, immunohistochemical (IHC) staining of tumour tissues indicated that ZG16 expression was significantly increased in the pLenti-ZG16 stably transfected LGR5+ CRC tumors, while significantly decreased in p-miR-196a stably transfected LGR5- CRC tumours (Figure 6E).