Still, before 5-Aza-treated CD4+CD25h T cells can be called “true” induced Treg cells, their transcription profile has to be compared to that of nTreg cells, and their suppressor function needs to be tested in vivo in an immunological setting such as the xenogeneic graft-versus-host disease (GvHD) model in NOD-scid-γc− (NSG) mouse (31). This evidence concerns the gene CD4 and graft versus host disease.