There was a prominent increase in the number of TUNEL-positive pancreatic cancer cells in Ad-TRAIL-CD40L/E1-CTL group as compared with the untreated control group, which indicate that Ad-TRAIL-CD40L/E1-CTL cells treatment could significantly increase tumor cell apoptosis as compared with untreated control cells. This evidence concerns the gene TNFSF10 and familial pancreatic carcinoma.