ZEB1 and esophageal cancer: We examined the protein levels of epithelial maker (ZO-1) and mesenchymal markers (N-cadherin, ZEB1, Slug, Snail) in esophageal cancer cells by immunoblotting analysis and found that the level of ZO-1 were markedly increased in KYSE70 and TE8 cells with the indicated concentrations of CYT-Rx20 compared with the control (P <0.05 in KYSE70; P <0.001 in TE8) (Fig 2C).