CD276 and infection: In fact, at least one bacterium was detectable in the cytosol of every cell 48h after infection in immunofluorescent stainings (Fig 8A, insertion) demonstrating that all cells had been in contact with R. typhi. We further analyzed the expression of MHCI, MHCII and costimulatory molecules on the cell surface 24h and 48h after inoculation.