PCR detection showed that ovariectomy led to a remarkable decrease in mRNA expression of BDNF, TrkB, CREB, ERK1 and ERK2 in both attacks and intervals of migraines induced by NTG infusion (P<0.05, Fig. 6), whereas restoration of serum estrogen level via estrogen administration reversed their mRNA expression. Here, MAPK3 is linked to migraine disorder.