With RT-PCR assay, mRNA expression of BDNF, TrkB, CREB and ERK (including the ERK1 and ERK2 subunits) were found to be significantly up-regulated in the brain of both female and male rats with migraine attack compared to non-migraine control rats (P<0.05, Fig. 2). The gene discussed is NTRK2; the disease is migraine disorder.