TNFRSF4 and neoplasm: To that end, splenocytes were isolated from mice vaccinated with C57MG DRibble plus anti-OX40 were either stimulated ex vivo or after culture in CM without exogenous cytokine for 5 days with 4T1-DRibbles pulsed onto spleen APC or whole 4T1 tumor cells (Fig. 2c–e).