SPRTN and progeroid features-hepatocellular carcinoma predisposition syndrome: To analyze how individual SPRTN mutations found in RJALS patients affect cell sensitivity to DPC-inducing agents, we created doxycycline (DOX) inducible and stable Flp-In HeLa cell lines, expressing wild-type (WT), enzymatic dead protein (E112A), and patient variants of SPRTN (Figures 2C–2H and S2C–S2F).