On the contrary, we observed that the normalized read count of the region corresponding to intron-1 was no different from the remaining introns of ARRB1 gene in control brain and GBM tissue samples (Additional file 4: Figure S1a, b), strongly suggesting that this intron did not give rise to any primary miRNA corresponding to miR-326 independently. The gene discussed is ARRB1; the disease is glioblastoma.