To investigate the anti-asthmatic effects of picroside II, we determined the levels of inflammatory cytokines, such as Th1 cytokine (IFNγ), Th2 cytokines (IL-4, IL-5, and IL-13), and asthma related cytokine (IL-33) in the BALF using ELISA kit. This evidence concerns the gene IL13 and asthma.