S2 Fig is an algorithm for analysis of activation and proliferation of PFMC and PBMC T cells. As shown in Fig 3A, PFMC CD4 T cells from HIV/TB subjects expressed significantly higher HLADR and CD38 double positive (HLADR/CD38), and CCR5 as compared to PBMC. Further, expression of CD69, and HLADR/CD38 were significantly higher on CD8 PFMC T cells than PBMC (Fig 3B). The gene discussed is CD4; the disease is tuberculosis.