Strikingly, the 240 genes in cluster 5 showed time-dependent upregulation and further upregulation under AI conditions (Figure 5b; Supplementary Figure S5), suggesting that these genes are gradually activated by NANOG and further upregulated, synergistically, by NANOG and castration and might also be involved in castration resistance and associated with PCa aggressiveness (Figure 5g). The gene discussed is NANOG; the disease is posterior cortical atrophy.