ChIP–quantitative PCR (qPCR) analysis in fresh samples against select peaks (Supplementary Figure S8A) identified by ChIP-Seq and using primers targeting these loci (Supplementary Table S7) revealed that, consistent with the ChIP-Seq data (Supplementary Figure S2C), both NANOG1 and NP8 occupied the promoter and enhancer regions of KLK3 gene and an enhancer in NKX3.1 gene under AD conditions (Figure 7b), and the occupancy was reduced in AI cells (that is, cells treated with 20 μm MDV3100 for 10 days; Figure 7c). Here, KLK3 is linked to Alzheimer disease.