On the other hand, NANOG binding to the enhancer region of UBE2C (Figure 3d; Supplementary Figure S8A), another cell cycle regulator implicated in PCa cell castration resistance [20], did not change in AD vs AI LNCaP cells (Figure 7b and c), although the UBE2C protein significantly increased in long-term castrated NANOG-expressing cells (Supplementary Figure S8C) similar to the increase in its mRNA levels under AI conditions (Figure 5h). Here, NANOG is linked to Alzheimer disease.