Thus, we took advantage of our established model of initially hormone-dependent LNCaP cells ectopically expressing DOX-inducible NP8 (or NANOG1 for comparison) [9] to perform genome-wide ChIP-Seq and transcriptome (RNA-Seq) analyses (Figure 2a) in a system that recapitulates PCa disease progression via NANOG-mediated reprogramming to castration resistance. Here, NANOG is linked to posterior cortical atrophy.