This is of functional relevance for asthma pathogenesis since selective inhibition of P2RX4 using 5-BDBD alleviates cardinal features of AAI, including airway eosinophilia, peribronchial inflammation, Th2 cytokine secretion of allergen re-stimulated MLN cells and BHR to increasing doses of inhaled methacholine in the murine model of OVA-induced airway inflammation. Here, P2RX4 is linked to asthma.