Interestingly, the expression of another EMT marker E-cadherin was not affected by either TGF-β1 or TGF-β inhibitor treatment, most likely because of the short-term treatment as it was reported by Maeda and coworkers [27] that the down-regulation of E-cadherin expression happened after TGF-β1 treatment for 4 days in cancer cells. This evidence concerns the gene TGFB1 and cancer.