It therefore seems likely that MEGF10 expression is silenced in most neuroblastomas by epigenetic mechanisms other than DNA methylation, such as repressive histone modifications H3K27me3 and/or H3K9me2, as we have shown in the SHIN cell line (Figure 4), and as has been reported for other neuroblastoma tumor suppressor genes 23, 24, 55. This evidence concerns the gene MEGF10 and neuroblastoma.