The transcriptional levels of inflammatory cytokines of TNF‐α (Fig. 5J), IL‐6 (Fig. 5K), IL‐1β (Fig. 5L) and COX‐2 (Fig. 5M) induced by ALI in lung tissue were also reduced by pre‐treatment with 20 mg/kg L2H21. This evidence concerns the gene TNF and acute respiratory distress syndrome.