The requirement of CPEB4 to maintain the expression of BUB1B, CDK1 and DEK was confirmed by protein immunoblotting after shRNA depletion in three cell lines from melanoma (SK-Mel-28, SK-Mel-103 and UACC-62), using other three non-melanoma lines (RWP1, HeLa and U251) as a reference (Supplementary Fig. 7a; note the modest or inconsistent downregulation of the analysed genes by CPEB4 depletion in the non-melanoma lines with respect to the downregulation in the melanoma cells). This evidence concerns the gene BUB1B and melanoma.