CCND1 and neoplasm: To further identify the contribution of Cyclin D1 ablation in berberine′s anti-tumor action, respectively, the plasmid encoding either HA-Cyclin D1 wt or HA-Cyclin D1 T286A were transfected into HepG2 cells followed by berberine treatment and WST-1 assay was used to detect the cell response to berberine.