VEGFA and peripheral arterial disease: The ratio VEGF165b:VEGF-A (from the densitometry values of western blot analysis) showed a ~4 fold induction in the fraction of VEGF165b in total VEGF-A in ischemic muscle at day 7 post HLI compared to non-ischemic (i.e. VEGF165b isoforms constitute ~80% of total VEGF-A) in experimental PAD muscle compared to control (Fig. 2).