In accordance to tumor genotyping, the BRAF V600E mutation was found in the basal cfDNA of patient 9 (0.07% of plasma DNA fragments), patient 12 (10.47%), patient 17 (0.40%) and patient 18 (3.64%), and the PIK3CA H1047R mutation was found in the cfDNA of patient 18 (2.62%) (Figure 2A). The gene discussed is PIK3CA; the disease is neoplasm.