To identify myogenic factors that are substrates of PRMT1, we designed a candidate peptide array with selected candidates containing methylation motifs in their sequence, including peptides from Pax3, Pax7, Myf5, MyoD, Myf6, Pitx2, Six1/4, and Eya1/2, as well as proteins related to adult muscle homeostasis/regeneration, skeletal muscle disorders, and potential substrates predicted to be methylated on PRMT1 preferred motifs (RGG/RG and RXR motifs) (Table 1). Here, EYA1 is linked to skeletal muscle disorder.