Subsequently, we collected pronephric cells from the Tg(wt1b:EGFP) zebrafish line (Fig. 2A) subjected to hyperglycaemia and performed a real-time quantitative PCR, to assess expressional changes of ELMO1 within the renal system of the zebrafish embryo under normo- and hyperglycaemic conditions. The gene discussed is ELMO1; the disease is Hyperglycemia.