As shown in Fig. 2C, YAP1 was dramatically downregulated in the detached cells compared to attached cells in MDA-MB-468 and several other epithelial cancer cell lines including MCF7 (luminal breast cancer), DU145 (prostate cancer) and HCT116 (colon cancer) with the exception of the mesenchymal prostate cancer cell line PC-3, which is known to be resistant to anoikis26. This evidence concerns the gene YAP1 and malignant colon neoplasm.