Furthermore, we examined the protein level of these inflammatory mediators in supernatants by ELISA, and the secreted protein level of IL-6, IL-1β, MMP1, and MMP13 was also suppressed in TRIP overexpressed RA-FLS compared with RA-FLS-Ctrl group after TNFα treated (Figure 3(b)). This evidence concerns the gene TNF and rheumatoid arthritis.