The most consistent expression gene, ATP1A1, in murine model and clinical specimens was further verified by qPCR in paired tumor and adjacent normal tissues of esophagus from 14 ESCC patients (Figure 2B and 2C).We found that ATP1A1 RNA was overexpressed in 10 (71.4%) out of 14 pairs, when tumor parts were compared to their adjacent normal parts (Figure 2B; details in Supplementary Table S2). This evidence concerns the gene ATP1A1 and neoplasm.