Inconsistent to the findings by Yuan et al. in BafA1- treated pediatric B-cell ALL cells, we observed that BafA1 treatment inhibits the activation of the class I PI3K/Akt/mTOR/p70S6K signaling pathway, suggesting that BafA1 treatment at low concentration may result in autophagy induction in HCC cells. The gene discussed is AKT1; the disease is hepatocellular carcinoma.