Exposure of cells to BafA1 over time induced robust LC3 conversion (i.e. cytosolic LC3I to autophagosome-bound lipidated LC3II) and increased p62 levels in both BEL7402 and HepG2 cells (Fig. 4B), thereby suggesting that BafA1 blocks autophagy flux in HCC cells. Here, SQSTM1 is linked to hepatocellular carcinoma.