Similarly in co-infection samples, one sample tested positive by CHIKV-IgM and DENV-IgM, three samples were positive by RT-PCR (CHIK), DENV-IgM and RT-PCR (DEN), five samples showed positive result by RT-PCR (CHIK) and DEN-IgM and all other samples were found to be positive by RT-PCR method for both the viruses. Here, CD40LG is linked to coinfection.