PCDHA4 and metastatic neoplasm: When we assessed PCDHA4 expression level by qRT-PCR 72 h after treatment of increasing doses of 5-Aza-dC we did not observe any change in ERMS cell lines (RD and RH36), but we did notice a dose dependent restoration of PCDHA4 expression in RH30, one of the ARMS cell lines representing the metastatic tumor (p < 0.05, Fig. 3b).