As shown in Figure 3a, the expression levels of the upstream molecules in this pathway, such as Kibra, FRMD6/Willin and Merlin/NF2, were upregulated or downregulated at both the transcriptional and protein levels with overexpressing or knocking down N3ICD in MDA-MB-231 breast cancer cells, respectively. This evidence concerns the gene FRMD6 and breast carcinoma.