In contrast, our experiments provide direct evidence of enhanced sensitivity to eNOS inhibition in caveolae-deficient outflow tissue and agree with a previous study on Cav-1-associated pulmonary hypertension where pulmonary arterial resistance was more dramatically increased by eNOS inhibition in Cav-1−/− than in control mice54. This evidence concerns the gene NOS3 and pulmonary arterial hypertension.