A significant decrease in renal klotho (ratio klotho/β-actin) was identified in all rats fed HF diets: rats with intact renal function fed NP, 0.75 ± 0.06 vs 0.97 ± 0.02 in rats fed NF diet (p < 0.01); 1/2Nx rats fed NP, 0.69 ± 0.07 vs 1.12 ± 0.08 in rats fed NF diet (p < 0.01); and rats with intact renal function fed HP, 0.57 ± 0.19 vs 1.16 ± 0.15 in rats fed NF diet (p < 0.05) (Fig. 2A). The gene discussed is KL; the disease is hydrops fetalis.