Splenocytes harvested from mice with/without ARDS may respond differently when cultured with varying levels of recombinant human sPD-1 we used a two-way analysis of variance to test the proliferative capacity of splenocytes from mice with and without ARDS when cultured with varying levels of recombinant sPD-1 (Fig. 3a). Here, HOXD13 is linked to acute respiratory distress syndrome.