As this work was initially performed to select the best Luminex kit for a TB/HIV study, PBMC from two healthy donors, one HIV-1-positive patient and one tuberculosis (TB)-positive patient were cultured in triplicate for 2 and 5 days without antigen (NS) or with PPD, early secreted antigenic target 6 kDa protein (ESAT-6) or SEB. Here, SETBP1 is linked to tuberculosis.