These findings were in accordance with the results of our previous studies that down-regulation of activity of CD4+CD25+Tregs through Astragalus Polysaccharides or high mobility group box (HMGB)-1 protein marked enhanced cell-mediated immunity by modulating the proliferation of CD4+CD25− T cells and the polarization of helper T cells, which was associated with improvement in outcome of burn-induced septic mice and decreasing the probability of secondary infection with P. aeruginosa [14–16]. The gene discussed is CD4; the disease is infection.